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KMID : 0359319940340030493
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Korean Journal of Veterinary Research
1994 Volume.34 No. 3 p.493 ~ p.500
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Actions of acetylcholine , norepinephrine , histamine and prostaglandin F2α , on motility of pig oviductal isthmic smooth muscle
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Abstract
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The purpose of this study was to investigate the effects of neurotransmitters and the source of Ca^(2+) in the effects of neurotransmitters on the motility of pig oviductal isthmic smooth muscle. The motility of the isolated smooth muscle was recorded by using physiological recording system.
The results were summarized as follows;
Acerylcholine, norepinephrine, histamine and prostaglandin F_(2α)(PGF_(2α)) caused the contraction and the contractile responses were increased in a dose-dependent manner from the concentration of 10^(-7) to 10^(-4) M. The maximum contractility of acetylcholine, norepinephrine, histamine and PGF_(2α) was 65.99, 28.66, 83.99 and 47.33% of l00 mM K contraction, respectively.
The contractile response induced by acetylcholine(10^(-6) M) was completely blocked by the pretreatment with cholinergic receptor blocker, atropine(10^(-6) M), the contractile reponse induced by norepinephrine(10^(-5) M) was blocked by the pretreatment with α-adrenergic receptor Mocker, phentolamine(10^(-6) M) but was not blocked and rather increased by the pretreatment with β-adrenergic receptor blocker. propranolol(10^(-6) M), the contractile response induced by histamine(10^(-6) M) was completely blocked by the pretreatment with H₁,-histaminergic receptor blocker, pyrilamine(10^(-6) M) but was increased by the pretreatment with H₂-histaminergic receptor blocker, cimetidine(10^(-6) M). The contractile response induced by acethylcholine(10^(-6) M), norepinephrine(10^(-5) M) and histamine(10^(-6) M) was weakly contracted response in Ca^(2+)-free medium, but the contractile response induced by PGF_(2α)(10^(-6) M) was disappeared. The contractile response induced by acerylcholine(10^(-6) M), norepinephrine(10^(-5) M) and histamine(10^(-6) M) was powerfully depressed by the pretreatment with Ca^(2+)-chafmel Mocker, verapamil(10^(-5) M) but the contractile response induced by PGF_(2α)(10^(-6) M) was completely inhibited.
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